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5–four μmol/L vary,[156][157] the focus of serotonin in synaptic vesicles was measured at 270 mM.[182] Following vesicular release, the resulting concentration of serotonin in the synaptic cleft, to which serotonin receptors are uncovered, is estimated for being about three hundred μmol/L. Consequently, although in vitro receptor binding affi

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